Network Directory

• Dr Becky Wilson, Consultant Microbiologist [email protected]
• Dr Noha El Sakka, Consultant Microbiologist [email protected]
• Lesley Beveridge, Laboratory Manager [email protected]
• Pauline Furness, BMS3 [email protected]
• Janette Rennie, BMS2 [email protected]
• Gary Stuart, BMS2 [email protected]

Direct immunofluorescence for respiratory viruses, HSV and VZV. EIA for rotavirus and adenovirus in faeces.

Real time PCR for HSV, VZV, CMV (quantitative), EBV (quantitative), adenovirus, enterovirus, norovirus and erythrovirus. The repertoire is in development.

A wide range of serology using automated, semi-automated and manual platforms.

Virology BMS are in the laboratory 0830 – 1700 Monday to Friday and 0830 to 1600 on Saturday.

Outwith these hours, the tests required for a potential organ donor transplant screen are available 24/7 by Microbiology BMS who are on site 24/7 and available via 01224 552451 or via the hospital switchboard 0845 4566000.

• Dr M Strutt, Consultant Microbiologist / Head of Service
• Dr G Calver, Consultant Microbiologist
• Dr J Nash, Consultant Microbiologist
• Dr S Reddy, Consultant Microbiologist
• Mrs Marie Wenham, Chief Biomedical Scientist
• Mr Marcus Coales, Head Biomedical Scientist [email protected]
• Mr John Skilton, Senior Biomedical Scientist

Virology
This section detects viruses by molecular methods.

Respiratory virus screen (includes Influenza A (+ H1, H3 and 2009 H1N1 typing), influenza B, Adenovirus, RSV, Parainfluenza 1,2,3 & 4, Rhinovirus, Coronavirus, Bocavirus, and enterovirus)

Meningitis screen: HSV-1, HSV-2, CMV, VZV, EBV, HHV6

Viral Loads: HIV-1, HCV, CMV

Serology

This section detects antibodies to viruses and bacteria, and viral antigens in serum/plasma using both manual and automated methods. Automated methods include: Abbott Architect (rubella, CMV, Toxoplasma, hepatitis A, B, C, Syphilis (all IgG and IgM), CMV Avidity HTLV-1/2, hepatitis C antibody, hepatitis B surface antigen and all markers and HIV (Ag/Ab).
bioMérieux mini Vidas (Lymes, Measles IgG, Mumps IgG, EBV IgG/M, VZV IgG, HBsAg and HIV duo)
Manual Methods include: Plate ELISA (VZV IgG, parvovirus IgG/M, HCV Ag/Ab, Mycoplasma), EIA card tests (HIV typing 1 or 2, cryptococcal antigen)

Immunofluorescence tests (Pneumocystis (carinii) jirovecii)
Any tests not covered within the laboratory repertoire are sent to the relevant reference laboratories.

Virology BMSs are available in the laboratory:

08:30-18:00 Tuesday – Thursday

08:30 – 17:00 Monday & Friday

08:30 – 12:15 Saturday

Outside of these hours virology advice can be obtained from the on-call Consultant Microbiologist available thorough the hospital switchboard.

• Dr. Stephanie Paget , Consultant Virologist [email protected]
• Linda Hodge, Service Manager [email protected]
• Juliet Marquez, Serology Specialist Scientist [email protected]

Routine Serology, Chlamydia and GC NAAT, HSV PCR, Helicobacter stools antigen, respiratory antigen tests, C diff testing

Urgent antenatal screening available via on-call

Key Link:

http://www.bcf.nhs.uk/intranet/pathology/services/microbiology/index?_ts=73528

• Dr CY William Tong , Consultant Virologist [email protected]
• Dr Zahir Babiker, Consultant Virologist [email protected]
• Dr Duncan Clark, Consultant Clinical Scientist and Honorary Senior Lecturer [email protected]
• Tony Oliver, Advanced Practitioner/Lead Biom edical Scientist [email protected]
• Xose CoutoParada, Laboratory Manager [email protected]
• Spiro Pereira, Laboratory Manager [email protected]
• Emily Kilpin, Laboratory Manager [email protected] NHS Net: [email protected]
• Jacquie Coombes, Divisional Administrator [email protected]
• Professor Rick Holliman, PHE Lead Public Health Microbiologist [email protected]
• Dr Gee Yen Shin, PHE Consultant Virologist [email protected]
• Julie Johnson, PHE Head of Operations [email protected]
• Dr Derren Ready, PHE Lead Clinical Scientist [email protected]

Diagnostic Virology at Barts Health NHS Trust provides a fully accredited service (CPA/ISO) for the timely diagnosis of viral infections and their clinical management. We are the HPA Lead Laboratory for London, providing Public Health testing and services for the capital.

Virology processes approximately 890,000 tests on 350,000 specimens annually and provides an extensive diagnostics service for the whole of the North East London Sector and beyond. Virology provides a full comprehensive range of diagnostic molecular, detection and serological assays in our state of the art Pathology building. Diagnostic results are augmented by clinical advice which is readily accessible both in and out of working hours.

We aim to be responsive to the needs of our users and in partnership with them have recently implemented a programme of development to introduce new methodology and systems. With these we aim to improve the timely diagnosis of newly emerging and established infectious diseases. This is particularly significant in the areas of molecular diagnostics and automated serology.

The Laboratory is approved by the Health Professions Council, the Institute of Biomedical Science, and the Royal College of Pathologists to conduct staff training in accordance with statutory registration of Professional and Regulatory bodies.

The laboratory is also the Lead Public Health Laboratory for London and provides support for national and local outbreaks as well as Public Health advice.

On call service outside routine hours
Links http:www.bartsandthelondon.nhs.uk/forclinicians

On Call Biomedical Scientist (B)

Mon-Fri 6pm to 8am, Sat/Sun 5pm to 9am

Air Call Pager 07659 810479 (via RLH switchboard 0207 377 7000)

For all clinical queries contact Virology/Microbiology Registrar in first instance

Daytime Duty Virology Consultant

Mon-Fri 09:00 to 17:00 out of hrs contact Virology/Microbiology Registrar

M: 07715 038336

Virology Specialist Registrar in Hours (C)

Virology Registrar (09:00 to 17:00)

M: 07715 038 333

Virology Specialist Registrar Out of Hours (D)

Virology/Microbiology Registrar (Out of Hours)

T: 020 3416 5000 (aircall)

• Professor Peter V Coyle, Consultant Virologist and Clinical Director Labs [email protected]; Tel: 02890638294
• Dr Conall McCaughey , Consultant Virologist [email protected]; Tel: 02890635239
• Dr. Tanya Curran , Clinical Scientist [email protected]; Tel: 0289063
• Dr. Susan Feeney , Clinical Scientist [email protected];Tel: 0289063
• Dr. Alison Watt , Clinical Scientist [email protected]; Tel: 0289063
• Dr Derek Fairley , Bioinformatician [email protected]
• Mr Mark McGimpsey , Operations Manager (Microbiology) [email protected]
• Mr Ian Doherty , Virology Laboratory Manager [email protected]
• Mr Eric Mitchell , Deputy Virology Laboratory Manager [email protected]

The laboratory provides regional molecular and serology services for Northern Ireland.

Web site: http://www.rvl-belfast.hscni.net/

User information:

http://www.rvl-belfast.hscni.net/wp-content/uploads/2014/05/Virology_User_Manual_2010.pdf

RVL website:

http://www.rvl-belfast.hscni.net/

• Dr Husam Osman, Consultant Virologist [email protected]
• Dr Erasmus Smit , Consultant Virologist [email protected]
• Dr Sowsan Atabani , Consultant Virologist [email protected]
• Dr Li Xu-McCrae, Lead Clinical Scientist [email protected]
• Jane Shirley , Operations Manager [email protected]
• Sarah Gardiner, Laboratory Manager [email protected]
• Dr Steven Wilson, Clinical Scientist [email protected]
• Judith Workmanr , Clinical Virology and Molecular Service Manage [email protected]

UKAS accredited laboratory that provides:

• Expert medical and scientific virological advice, including access to PHE experts locally and nationally as necessary
• A wide range of diagnostic, specialist and reference tests
• Essential diagnostic PCRs performed daily including weekends and public holidays
• Electronic results available via elabs and/or iGateway
• National standard methods and PHE testing algorithms
• The laboratory provides support for the investigation, management and control of infection and outbreaks of communicable diseases both during and out of normal working hours including measles
• One of the largest centres within the UK for antiviral susceptibility testing
• One of only two centres within the UK for the investigation and monitoring of HBV infected healthcare workers
• One of only two centres within England for the investigation of MERS cases

Key Links

http://www.heftpathology.com/index.php?option=com_content&view=category&layout=blog&id=46&Itemid=130

• Dr Mohammed Osman Hassan‐Ibrahim, Consultant Virologist [email protected]
• Mr Gary Homer, Senior Biomedical Scientist (8a) [email protected]
• Mr Nicholas O'Flanagan, Senior Biomedical Scientist (Band 7) [email protected]
• Mrs Charlotte Stubbs , Molecular Specialist Scientist [email protected]
• Mr Nicholas O’Flanagan , Serology Specialist Scientist [email protected]

Blood borne viruses (quantitative):
HIV viral load
HBV viral load
HCV viral load
Respiratory multiplex panel:
Influenza (A & B) RNA
Parainfluenza (1‐4) RNA
RSV RNA
Human metapneumovirus RNA
Adenovirus DNA
Rhinovirus RNA
Enterovirus RNA
Parechovirus RNA
Human bocavirus DNA
Coronaviruses (NL63/OC43/229E/HKU) RNA
Mycoplasma pneumoniae nucleic acid
CSF multiplex panel:
Herpes Simplex Virus types 1 & 2
Varicella zoster virus
Enterovirus RNA
Mumps RNA
Gastroenteritis multiplex panel:
Norovirus (G1 & G2) RNA
Rotavirus RNA
Adenovirus RNA
Astrovirus RNA

Fever & rash multiplex panel:
Measles RNA
Enterovirus RNA
Parvovirus B19 DNA
Human herpes virus type 6 DNA
Human herpes virus type 7 DNA

Transplant multiplex panel (quantitative):
CMV DNA
EBV DNA
Adenovirus DNA
Ophthalmology multiplex panel:
Herpes Simplex Virus types (1 & 2) DNA
Varicella zoster virus DNA
Adenovirus DNA
Chlamydia trachomatis nucleic acid
Chlamydia & GC NAAT:
Chlamydia nucleic acid
Gonorrhoea nucleic acid

Other individual molecular tests:
Mumps RT‐PCR
HSV‐1 & HSV‐2 PCR
VZV PCR
CMV PCR (quantitative)
EBV PCR (quantitative)
Influenza RT‐PCR
Adenovirus PCR
Parvovirus B19 PCR
Chlamydia PCR

Serology (including antigen & POCT tests):
HIV serology:
HIV Ag/Ab Combo
HIV VIDAS DUO Ultra
HIV immunocomb
HAV (IgM & IgG) serology
HBV serology:
HBsAg & neutralisation
Anti‐HBc total & Anti‐HBc IgM
HBeAg & Anti‐HBe
Anti‐HBs
HCV serology:
Hep C antibody
Hep C Ag/Ab
HEV (IgM & IgG) serology [under review]
HTLV serology
Syphilis serology:
RPR
HSV‐1 & HSV‐2 (IgG type specific) serology
VZV (IgM & IgG) serology
CMV (IgM, IgG, avidity) serology
EBV serology:
VCA IgM
EBNA IgG
VCA IgG
Rubella (IgM & IgG) serology
Measles (IgM & IgG) serology
Mumps (IgM & IgG) serology
Parvovirus B19 (IgM & IgG) serology
ASO titre
Toxoplasma (IgM & IgG) serology
Lyme total antibody serology
H. pylori antibody serology Legionella & pneumococcal urine antigen testing
Cryptococcal antigen testing

http://pathology.bsuh.nhs.uk/pathology/MicrobiologyInfection/tabid/57/Default.aspx

• Dr Matthew Donati, Consultant Virologist & Clinical Service Director [email protected]
• Dr Peter Muir, Consultant Clinical Scientist & Head of Virology [email protected]
• Dr Javeed Ahmed, Consultant Virologist [email protected]
• Dr Charles Irish, Consultant Virologist [email protected]
• Dr Barry Vipond, Clinical Scientist & Head of Molecular Diagnostics
• Mr Will Gothard, Head of Operations [email protected]
• Miss Jane Usher, Deputy Laboratory Manager (Virology) [email protected]
• Mrs Lis North, Deputy Laboratory Manager (Virology) [email protected]

Public Health and Clinical Virology, molecular diagnostics, infectious disease serology

24/7 service via Bristol Royal Infirmary switchboard (01179230000)

• Dr Nicholas Brown, Consultant Medical Microbiologist, Interim Lead Public Health Microbiologist, East of England & Director [email protected]
• Dr Hongyi Zhang , Consultant Virologist & Deputy Director [email protected]
• Ms Laura Ryall , Regional Head of Operations, PHE East of England [email protected]
• Dr. Hamid Jalal , Consultant Virologist [email protected]
• Dr Kathryn Rolfe, Virology Consultant Clinical Scientist [email protected]
• Dr Chris Smith , Consultant Virologist [email protected]
• Dr Tim Wreghitt , Virology Consultant Clinical Scientist [email protected]
• Dr Martin Curran, Head of Molecular Development [email protected]
• Dr Jane Greatorex, Senior Scientist [email protected]
• Mr Martin Strickland , Microbiology Laboratory Manager [email protected]
• Mr Martin L’Estrange, Virology/Molecular Service Manager [email protected]
• Mr Surendra Parmar, Molecular Development Scientist [email protected]
• Mr Gordon Sutehall, Governance Manager [email protected]
• Marianne Milbourne , Administration Manager [email protected]

We offer comprehensive diagnostic serology and molecular services for viral and microbial pathogens complete with clinical interpretation and advice.

Serology Tests

Hepatitis A virus total and IgM antibodies

Hepatitis B virus screening, confirmation and markers

Hepatitis C virus screening and confirmation

Hepatitis D virus antibodies

Hepatitis E virus IgG and IgM

HIV screening and confirmation

CMV IgG and IgM

Erythrovirus IgG and IgM

EBV IgG and IgM

Measles virus IgG and IgM

Mumps virus IgG and IgM

Rubella virus IgG and IgM

HTLV I/II antibodies

VZV IgG and IgM

HSV IgG

Toxoplasma gondii IgG

Helicobacter pylori faecal antigen

Helicobacter pylori IgG

Treponema pallidum IgG

Borrelia burgdorferi IgG

Streptococcal serology

Rotavirus/adenovirus faecal antigen

Molecular Tests

Respiratory virus multiplex PCR for influenza A, influenza B, RSV, rhinovirus, metapneumovirus, adenovirus and parainfluenza virus types 1-4.

Influenza A virus subtyping (H1, H3, H5, H7) and oseltamivir susceptibility testing of H1N1.

Quantitative PCR assays for CMV, HIV, HBV, HCV, BKV, EBV and adenovirus.

Qualitative PCR assays for EBV, adenovirus, norovirus, enteroviruses, parechovirus, JCV, HEV, HSV-1, HSV-2, VZV, measles, Bordetella pertussis and Pneumocystis jirovecii.

Genotyping of HCV and HIV

Genotypic resistance testing of HIV

NAAT for Chlamydia trachomatis and Neisseria gonorrhoeae

Ribotyping of Clostridium difficile

Out of hours’ service

Urgent testing for blood-borne viruses following needle-stick injury.

Urgent testing for blood-borne viruses in organ donors.

Key Links:

PHE Website

www.gov.uk/government/organisations/public-health-england

Addenbrookes Website

www.cuh.org.uk/addenbrookes-hospital

• Dr. Rachel Jones, Consultant Virologist National Lead [email protected]
• Dr. Nicola Price, Consultant Virologist [email protected]
• Dr. Laura Dexter , Consultant Virologist [email protected]
• Dr. Jaisi Sinha , Consultant Microbiologist / Virologist [email protected]
• Mr Jonathan Evans, Operations Manager [email protected]
• Dr Sally Corden, Head of Welsh Molecular Unit [email protected]
• Dr Catherine Moore, Clinical Scientist [email protected]
• Dr Neil Owen, Quality Lead Virology [email protected]
• Dr. Bassam Ben Ismaeil, Consultant Microbiologist / Virologist [email protected]
• Dr Mike Isaac, Clinical Scientist Head of Unit [email protected]
• Jenny Bayliss , Senior Biomedical Scientist [email protected]

Virology diagnostic services in Wales are provided as a Public Health Wales Virology Network.  The network’s main reference laboratory is the Wales Specialist Virology Centre (WSVC) based in University Hospital Wales – Cardiff along with the Specialist Virology Unit (WSVU)  based in Singleton Hospital – Swansea.

The Virology Diagnostic Network works in collaboration with the Molecular Diagnostic Unit (based in University Hospital Wales – Cardiff) to respond to emerging health needs (such as measles and MERS coronavirus) and to deliver virological surveillance and screening programmes across Wales. Both laboratories hold CPA accreditation as part of the Public Health Wales accredited laboratory network and participate in national IQC and EQA schemes with regular clinical governance meetings.

Under the guidance of WSVC the Public Health Wales Virology Network is committed to the reliable delivery and continuing development of a high quality, standardised All-Wales seromolecular service for the diagnosis and management of virological infection. It also supports the many other functions of Public Health Wales in the provision of an evidence-based, reactive service for infectious diseases in Wales.

WSVC has 30 staff members performing approximately 400,000 tests on 180,000 samples per annum, employing more than 100 different laboratory tests.  The WSVC offers virology clinical advice and urgent testing 24 hours a day, 7 days a week, serving both local and national needs.

WSVU – Swansea has 13 staff members performing approximately 285,000 tests on 150,000 samples per annum, employing more than 140 different laboratory tests.  The WSVU offers virology clinical advice and urgent testing 24 hours a day, 7 days a week, serving local needs.